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KMID : 1134820200490080848
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 8 p.848 ~ p.854
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Determination of Agricultural Pesticides in Raw Material of Milk with High Performance Liquid Chromatography and Tandem Mass Spectrometry
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Han Yu-Gyeong
Choi Young-Nae
Kang Gyeong-Won
Kim Jung-Nyen
Kim Jung-Bok
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Abstract
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Carbamate pesticides are compounds obtained by the reaction of carbamic acid and amine. These pesticides are often used as insecticides and herbicides. Especially, bendiocarb, carbaryl and carbofuran are popular carbamate pesticides LC-MS/MS using liquid-liquid extraction (LLE) and C18 cartridges was used to identify and quantity the levels of bendiocarb, carbaryl and carbofuran in raw material milk with high sensitivity and specificity. A calibration curve was obtained from 5 to 100 ng/mL with a satisfactory correlation coefficient of 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) for bendiocarb, carbaryl and carbofuran in the matrix were 0.8 ng/mL to 1.5 ng/mL and 3.0 ng/mL to 6.0 ng/mL, respectively. The recovery of the three pesticides from spiked samples at 15 ng/mL ranged from 103.6 to 110.7% with a relative standard deviation (RSD) between 4.6 to 4.9%. The measurement of uncertainty of the LC-MS/MS method developed for the three pesticides was based on the analytical process and quantification. Thus, the current study has established a fase and easy method for the analysis of bendiocarb, carbaryl and carbofuran in raw material milk by LC-MS/MS.
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KEYWORD
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carbamate pesticides, milk, method validation, C18 cartridge, LC-MS/MS
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